Work

Processing of hepatocyte growth factor to the heterodimeric form is required for biological activity

Year: 1992

Type: article

Abstract: Hepatocyte growth factor is a plasminogen-like molecule with diverse biological effects. Although it is synthesized as a single chain polypeptide, it was originally purified as a disulfide-linked heterodimer which was generated by an internal proteolytic event. Subsequent work indicated that preparations consisting largely of the monomeric form also exhibited potent activity. By using a combination of protease inhibition and site-directed mutagenesis, we established that conversion of the single chain polypeptide to the heterodimer occurred during the bioassay and was required for mitogenic and motogenic activity.

Source: FEBS Letters

DOI: 10.1016/0014-5793(92)81356-q

Authors: Eva Gak, William G. Taylor, Andrew M.-L. Chan, Jeffrey S. Rubin

Institutions: National Institutes of Health, National Cancer Institute

Language: English

Topic: Liver physiology and pathology

Subfield: Hepatology

Field: Medicine

Domain: Health Sciences

Open Access status: bronze

OpenAlex ID: W2019350457

FWCI: 2.74

Cites: 38

Cited by: 84

Related to: 10