Work

Fluorescent dyes as probes to study lipid‐binding proteins

Year: 2003

Type: article

Abstract: We studied the equilibrium binding of two hydrophobic fluorescent dyes, ANS and bisANS, to four members of a family of intracellular lipid-binding proteins: IFABP, CRABP I, CRABP II, and ILBP. The spectral and binding parameters for the probes bound to the proteins were determined. Typically, there was a single binding site on each protein for the ligands. However, IFABP cooperatively bound a second bisANS molecule in the binding pocket. Comparative analysis of affinities and spectral characteristics for the two probes allowed us to examine the contributions of electrostatic and hydrophobic interactions to the binding process, and to address some aspects of the internal structure of the studied proteins.

Source: Proteins Structure Function and Bioinformatics

DOI: 10.1002/prot.10401

Authors: Alexander V. Pastukhov, Ira J. Ropson

Institution: Pennsylvania State University

Language: English

Topic: Protein Interaction Studies and Fluorescence Analysis

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2019656619

FWCI: 0.462

Cites: 29

Cited by: 50

Related to: 10