Work

Analysis of the Extracts of Isatis tinctoria by New Analytical Approaches of HPLC, MS and NMR

Year: 2011

Type: article

Abstract: The methods of extraction, separation and analysis of alkaloids and indole glucosinolates (GLs) ofIsatis tinctoria were reviewed. Different analytical approaches such as High-pressure Liquid Chromatography (HPLC), Liquid Chromatography with Electrospray Ionization Mass Spectrometry (LC/ESI/MS), Electrospray Ionization Time-Of-Flight Mass Spectrometry (ESI-TOF-MS), and Nuclear Magnetic Resonance (NMR) were used to validate and identity of these constituents. These methods provide rapid separation, identification and quantitative measurements of alkaloids and GLs of Isatis tinctoria. By connection with different detectors to HPLC such as PDA, ELSD, ESI- and APCI-MS in positive and negative ion modes, complicated compounds could be detected with at least two independent detection modes. The molecular formula can be derived in a second step of ESI-TOF-MS data. But for some constituents, UV and MS cannot provide sufficient structure identification. After peak purification, NMR by semi-preparative HPLC can be used as a complementary method.

Source: African Journal of Traditional Complementary and Alternative Medicines

DOI: 10.4314/ajtcam.v8i5s.13

Authors: J Zhou, F Qu

Institutions: Zhejiang Gongshang University, King's College London, Women's Hospital, School of Medicine, Zhejiang University, Zhejiang University

Language: English

Topic: Traditional Chinese Medicine Analysis

Subfield: Complementary and alternative medicine

Field: Medicine

Domain: Health Sciences

Open Access status: diamond

Funders: National Natural Science Foundation of China, China Postdoctoral Science Foundation

Award: FSHR在绝经后脂肪细胞增殖、分化和代谢中的作用机制研究