Work

Selection and identification of single domain antibody fragments from camel heavy‐chain antibodies

Year: 1997

Type: article

Abstract: Functional heavy-chain gamma-immunoglobulins lacking light chains occur naturally in Camelidae. We now show the feasibility of immunising a dromedary, cloning the repertoire of the variable domains of its heavy-chain antibodies and panning, leading to the successful identification of minimum sized antigen binders. The recombinant binders are expressed well in E. coli, extremely stable, highly soluble, and react specifically and with high affinity to the antigens. This approach can be viewed as a general route to obtain small binders with favourable characteristics and valuable perspectives as modular building blocks to manufacture multispecific or multifunctional chimaeric proteins.

Source: FEBS Letters

DOI: 10.1016/s0014-5793(97)01062-4

Authors: M Arbabi Ghahroudi, A Desmyter, L Wyns, R Hamers, S Muyldermans

Institution: Vrije Universiteit Brussel

Language: English

Topic: Monoclonal and Polyclonal Antibodies Research

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

Open Access status: bronze

Funder: Fonds Wetenschappelijk Onderzoek

OpenAlex ID: W2020368956

FWCI: 3.459

Cites: 29

Cited by: 790

Related to: 10