Work

Reverse Genetics Analysis of Poxvirus Intermediate Transcription Factors

Year: 2012

Type: article

Abstract: Vaccinia virus transcription is regulated in three stages. An intermediate transcription factor, comprised of virus-encoded polypeptides A8 and A23, was previously identified by in vitro analyses. To investigate its role, we engineered cells that stably expressed both subunits and complemented the replication of A8 and A23 deletion mutant viruses. Without A8 or A23, viral early gene expression and DNA replication occurred but intermediate and late gene expression and resolution of genome concatemers were not detected.

Source: Journal of Virology

DOI: 10.1128/jvi.06902-11

Authors: Robin D. Warren, Catherine A. Cotter, Bernard Moss

Institutions: National Institutes of Health, National Institute of Allergy and Infectious Diseases

Language: English

Topic: Poxvirus research and outbreaks

Subfield: Virology

Field: Immunology and Microbiology

Domain: Life Sciences

Open Access status: green

Funder: National Institutes of Health

OpenAlex ID: W2021281389

FWCI: 0.658

Cites: 25

Cited by: 28

Related to: 10