Work

A novel mediator between activator proteins and the RNA polymerase II transcription apparatus

Year: 1990

Type: article

Abstract: One gene activator protein may interfere with the effects of another in eukaryotic cells. We report here that a hybrid yeast-herpes gene activator protein inhibits transcriptional activation by a thymidine-rich DNA element in yeast. This example of activator interference can be faithfully reproduced in vitro. Interference is reversed by a partially purified yeast component, but not by RNA polymerase II or various polymerase II transcription factors. We conclude that the partially purified yeast component is a novel factor, and we suggest this factor mediates the transcriptional activation process.

Source: Cell

DOI: 10.1016/0092-8674(90)90685-8

Authors: Raymond J. Kelleher, Peter M. Flanagan, Roger D. Kornberg

Institutions: Stanford Medicine, Stanford University

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $11,400

Funders: National Institutes of Health, National Institute of General Medical Sciences