Work

Live Cell Interferometry Reveals Cellular Dynamism During Force Propagation

Year: 2008

Type: article

Abstract: Cancer and many other diseases are characterized by changes in cell morphology, motion, and mechanical rigidity. However, in live cell cytology, stimulus-induced morphologic changes typically take 10-30 min to detect. Here, we employ live-cell interferometry (LCI) to visualize the rapid response of a whole cell to mechanical stimulation, on a time scale of seconds, and we detect cytoskeletal remodeling behavior within 200 s. This behavior involved small, rapid changes in cell content and miniscule changes in shape; it would be difficult to detect with conventional or phase contrast microscopy alone and is beyond the dynamic capability of AFM. We demonstrate that LCI provides a rapid, quantitative reconstruction of the cell body with no labeling. This is an advantage over traditional microscopy and flow cytometry, which require cell surface tagging and/or destructive cell fixation for labeling.

Source: ACS Nano

DOI: 10.1021/nn700303f

Authors: Jason Reed, Joshua J. Troke, Joanna Schmit, Sen Han, Michael A. Teitell, James K. Gimzewski

Institutions: California NanoSystems Institute, University of California, Los Angeles, Veeco (United States), UCLA Jonsson Comprehensive Cancer Center

Language: English

Topic: Digital Holography and Microscopy

Subfield: Atomic and Molecular Physics, and Optics

Field: Physics and Astronomy

Domain: Physical Sciences

Open Access status: green

Funders: National Institutes of Health, National Eye Institute, National Cancer Institute, National Institute of General Medical Sciences

Awards: A NOVEL MECHANISM OF TCL1 TUMORIGENESIS, RNA trafficking in mitochondria, The Center for Systemic Control of Cyto-Networks, CRTC2 in Cellular Development, Function, and Neoplasia, Nano mirrors for high throughput cell motion analysis