Year: 2009
Type: article
Abstract: BACKGROUND: Chondrogenesis and subsequent endochondral ossification are processes tightly regulated by the transcription factor Sox9 (SRY-related high mobility group-Box gene 9), but molecular mechanisms underlying this activity remain unclear. Here we report that coactivator-associated arginine methyltransferase 1 (CARM1) regulates chondrocyte proliferation via arginine methylation of Sox9. RESULTS: CARM1-null mice display delayed endochondral ossification and decreased chondrocyte proliferation. Conversely, cartilage development of CARM1 transgenic mice was accelerated. CARM1 specifically methylates Sox9 at its HMG domain in vivo and in vitro. Arg-methylation of Sox9 by CARM1 disrupts interaction of Sox9 with beta-catenin, regulating Cyclin D1 expression and cell cycle progression of chondrocytes. CONCLUSION: These results establish a role for CARM1 as an important regulator of chondrocyte proliferation during embryogenesis.
Source: BMC Developmental Biology
Authors: Tatsuo Ito, Neelu Yadav, Jaeho Lee, Takayuki Furumatsu, Satoshi Yamashita, Kenji Yoshida, Noboru Taniguchi, Megumi Hashimoto, Megumi Tsuchiya, Toshifumi Ozaki, Martin Lotz, Mark T Bedford, Hiroshi Asahara
Language: English
Subfield: Rheumatology
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $2,290
Funders: Memorial Sloan-Kettering Cancer Center, Tokyo Medical and Dental University, National Institutes of Health, University of California, San Francisco, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Center for Research Resources, School of Medicine, University of California, San Francisco, National Institute for Basic Biology
Awards: The role of CARM1 in chondrogenesis, GENERAL CLINICAL RESEARCH CENTER, The Functional Analysis of the Coactivator CARM1, The Functional Analysis of the Coactivator CARM1, The role of microRNAs in cartilage homeostasis and osteoarthritis arthritis treatment, G6626709839, G6840756229, G7815799437, G9000067774, G9000187776, G9000196954
OpenAlex ID: W2022200846