Work

Class I molecules retained in the endoplasmic reticulum bind antigenic peptides.

Year: 1993

Type: article

Abstract: We isolated major histocompatibility complex (MHC)-specific viral peptides from cells infected with influenza virus in the continuous presence of the drug brefeldin A, which blocks exocytosis of newly synthesized MHC class I molecules. MHC-specific peptides were also isolated from cells expressing mouse Kd class I MHC molecules whose cytoplasmic domain was substituted by that of the adenovirus E3/19K glycoprotein. This molecule was retained in an intracellular pre-Golgi complex compartment as demonstrated by immunocytochemical and biochemical means. Since we show that intracellular association of antigenic peptides with such retained class I molecules is necessary for their isolation from cellular extracts, this provides direct evidence that naturally processed peptides associate with class I MHC molecules in an early intracellular exocytic compartment.

Source: The Journal of Experimental Medicine

DOI: 10.1084/jem.177.6.1633

Authors: C K Lapham, I Bacík, J W Yewdell, K P Kane, J R Bennink

Institutions: National Institutes of Health, National Institute of Allergy and Infectious Diseases

Language: English

Topic: vaccines and immunoinformatics approaches

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: Cancer Research Institute, Fondation pour la Recherche Médicale, National Institutes of Health, National Institute of Allergy and Infectious Diseases