Year: 2010
Type: article
Abstract: Thrombus development in mouse mesenteric vessels following laser-induced injury was monitored by high-resolution, near-real-time, two-photon, intravital microscopy. In addition to the use of fluorescently tagged fibrin(ogen) and platelets, plasma was labeled with fluorescently tagged dextran. Because blood cells exclude the dextran in the single plane, blood cells appear as black silhouettes. Thus, in addition to monitoring the accumulation of platelets and fibrin in the thrombus, the protocol detects the movement and incorporation of unlabeled cells in and around it. The developing thrombus perturbs the blood flow near the thrombus surface, which affects the incorporation of platelets and blood cells into the structure. The hemodynamic effects and incorporation of blood cells lead to the development of thrombi with heterogeneous domain structures. Additionally, image processing algorithms and simulations were used to quantify structural features of developing thrombi. This analysis suggests a novel mechanism to stop the growth of developing thrombus.
Source: Journal of Biomedical Optics
DOI: 10.1117/1.3322676
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
Awards: Computational Geometry Algorithms for Medical Problems in Radiation Therapy and Medical Imaging, G1800464533, G2419578711, Deep LOGISMOS, Pathophysiologies Involving Hemostasis-related Genes, Integrating Multiscale Modeling and in vivo Experiments for Studying Blood Clot Development, AF: Small: Algorithmic Problems in Applied Computational Geometry, G9000017215, G9000103092
OpenAlex ID: W2022443343