Work

Identification of a vaccinia virus gene encoding a type I DNA topoisomerase.

Year: 1987

Type: article

Abstract: Vaccinia virus encapsidates a type I DNA topoisomerase (EC 5.99.1.2). The enzyme was purified from virus cores to apparent homogeneity, yielding a protein of Mr 32,000. The amino-terminal sequence of the isolated Mr 32,000 polypeptide was determined and used to map the putative structural gene for the vaccinia topoisomerase to the H7r open reading frame of the vaccinia genome. This gene encodes a 314-amino acid polypeptide containing a region homologous to a region of the type I topoisomerase from the yeast Saccharomyces cerevisiae.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.84.21.7478

Authors: S Shuman, B Moss

Institution: National Institute of Allergy and Infectious Diseases

Language: English

Topic: Poxvirus research and outbreaks

Subfield: Virology

Field: Immunology and Microbiology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2022493864

FWCI: 2.844

Cites: 19

Cited by: 152

Related to: 10