Work

CAP-T cell expression system: a novel rapid and versatile human cell expression system for fast and high yield transient protein expression

Year: 2011

Type: article

Abstract: BackroundCAP (CEVEC's Amniocyte Production) cells are an immortalized cell line based on primary human amniocytes.They were generated by transfection of these primary cells with a vector containing the functions E1 and pIX of adenovirus 5. CAP cells allow for competitive stable production of recombinant proteins with excellent biologic activity and therapeutic efficacy as a result of authentic human posttranslational modification.In order to gain access to the benefits of the CAP technology also for early research, target evaluation or assay development, the transient expression system CAP-T was developed.CAP-T cells are based on the original CAP cells and additionally express the large T antigen of simian virus 40 (SV40).

Source: BMC Proceedings

DOI: 10.1186/1753-6561-5-s8-p133

Authors: Jens Wölfel, Ruth Essers, Corinna Bialek, Sabine Hertel, Nadine Scholz-Neumann, Gudrun Schiedner

Institution: Federal Office of Administration

Language: English

Topic: Viral Infectious Diseases and Gene Expression in Insects

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,290