Evidence that Fusidic Acid Inhibits the Binding of Aminoacyl‐tRNA to the Donor as well as the Acceptor Site of the Ribosomes
Year: 1973
Type: article
Abstract: A relatively high concentration of fusidic acid (3 mM) inhibits the binding of aminoacyl‐tRNA to the donor as well as the acceptor site of ribosomes in the absence of elongation factor G (EF‐G). In the presence of EF‐G a low concentration of fusidic acid (1 mM) inhibited the nonenzymatic binding of aminoacyl‐tRNA to the donor and the acceptor site approximately 30% and 80%, respectively. On the other hand, binding of aminoacyl‐tRNA to the acceptor site which is dependent on elongation factor T, was specifically inhibited by, EF‐G, GTP and fusidic acid. From these observations, it is suggested that the binding site of fusidic acid, GDP and EF‐G may not be strictly located at the acceptor site.
Source: European Journal of Biochemistry
Authors: Tadahiko Otaka, Akira Kaji
Institution: University of Pennsylvania
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W2023200847