Work

Exact Analysis of Ligand-Induced Dimerization of Monomeric Receptors

Year: 2008

Type: article

Abstract: This paper analyzes the equilibria involved in the dimerization of monomeric receptors with homo-bifunctional ligands. We provide analytical expressions that can be used to estimate the concentration of each species present in a mixture of homo-bifunctional ligand and monomeric proteins, given initial conditions defining the total concentration of bivalent ligand [L2]0, the total concentration of protein [P]0, one dissociation constant Kd, and a parameter to account for cooperativity alpha. We demonstrate that the fraction of protein present in a complex of two proteins and one bivalent ligand (P x L2 x P) is maximized at [L2]0 = Kd/2 + [P]0/2.

Source: Analytical Chemistry

DOI: 10.1021/ac800578w

Authors: Eric T. Mack, Raquel Perez-Castillejos, Zhigang Suo, George M. Whitesides

Institution: Harvard University

Language: English

Topic: Receptor Mechanisms and Signaling

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of General Medical Sciences

Awards: G2080624118, ENZYME CATALYZED SYNTHESIS OF MONOSACCHARIDES, Multivalency: Mechanisms and Applications, G5240773006, Carbonic Anhydrase as a Model to Understand Divalency and Multivalent Linkers