Work

Fluorous enzymatic synthesis of phosphatidylinositides

Year: 2014

Type: article

Abstract: A fluorous tagging strategy coupled with enzymatic synthesis is introduced to efficiently synthesize multiple phosphatidylinositides, which are then directly immobilized on a fluorous polytetrafluoroethylene (PTFE) membrane to probe protein-lipid interactions.

Source: Chemical Communications

DOI: 10.1039/c4cc00022f

Authors: Weigang Huang, Angela Proctor, Christopher E. Sims, Nancy L. Allbritton, Qisheng Zhang

Institution: University of North Carolina at Chapel Hill

Language: English

Topic: Lipid Membrane Structure and Behavior

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Cancer Institute, National Institute of General Medical Sciences

Awards: Multiplexed Measurement of Kinase Activity in Single Cancer Cells, G2941629260, G564908764, High-throughput screens to identify modulators of phospholipase C isozymes