Work

DNA-Mediated Assembly of Protein Heterodimers on Membrane Surfaces

Year: 2013

Type: article

Abstract: We present a method based on self-assembling oligonucleotides to anchor proteins to a supported membrane surface. This anchoring method allows control of the surface density of multiple proteins. By incorporating additional recognition sequences into the DNA linkers, defined heterodimers can be produced upon the addition of a heterospecific DNA cross-linking strand. Characterization by fluorescence cross-correlation spectroscopy (FCCS) confirmed lateral mobility and the formation of specific heterodimers. We further demonstrate that proteins linked in this manner as either monomers or dimers can form functional interfaces with living cells.

Source: Journal of the American Chemical Society

DOI: 10.1021/ja3101215

Authors: Michael P. Coyle, Qian Xu, Samantha Chiang, Matthew B. Francis, Jay T. Groves

Institutions: Lawrence Berkeley National Laboratory, Howard Hughes Medical Institute, University of California, Berkeley

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $4,500

Funders: Howard Hughes Medical Institute, U.S. Department of Energy, National Institutes of Health, U.S. Public Health Service