Work

Linkage of recognition and replication functions by assembling combinatorial antibody Fab libraries along phage surfaces.

Year: 1991

Type: article

Abstract: We describe a method based on a phagemid vector with helper phage rescue for the construction and rapid analysis of combinatorial antibody Fab libraries. This approach should allow the generation and selection of many monoclonal antibodies. Antibody genes are expressed in concert with phage morphogenesis, thereby allowing incorporation of functional Fab molecules along the surface of filamentous phage. The power of the method depends upon the linkage of recognition and replication functions and is not limited to antibody molecules.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.88.10.4363

Authors: A S Kang, C F Barbas, K D Janda, S J Benkovic, R A Lerner

Institution: Scripps Research Institute

Language: English

Topic: Monoclonal and Polyclonal Antibodies Research

Subfield: Radiology, Nuclear Medicine and Imaging

Field: Medicine

Domain: Health Sciences

Open Access status: green

OpenAlex ID: W2024142971

FWCI: 51.73

Cites: 23

Cited by: 411

Related to: 10