Year: 2011
Type: article
Abstract: The design and fabrication of a membrane-integrated microfluidic cell culture device (five layers,≤500 μm total thickness) developed for high resolution microscopy is reported here. The multi-layer device was constructed to enable membrane separated cell culture for tissue mimetic in vitro model applications and pharmacodynamic evaluation studies. The microdevice was developed via a unique combination of low profile fluidic interconnect design, substrate transfer methodology, and wet silane bonding. To demonstrate the unique high resolution imaging capability of this device, we used oil immersion microscopy to image stained nuclei and mitochondria in primary hepatocytes adhered to the incorporated membrane.
Source: Biomicrofluidics
DOI: 10.1063/1.3647824
Authors: Alla A. Epshteyn, Steven Maher, Amy J. Taylor, Angela B. Holton, Jeffrey T. Borenstein, Joseph D. Cuiffi
Institutions: Draper Laboratory, University of South Florida
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W2024686482