Work

Polymerase Chain Reaction‐Restriction Fragment Length Polymorphisms (PCR‐RFLP) and Electrophoretic Assays for the Mouse Obese (Lepob) Mutation

Year: 1997

Type: article

Abstract: Three polymerase chain reaction-based assays for the mouse Lepob mutation are described: Dde I site created by the C-->T transversion characterizing Lepob enables positive detection of the mutant allele; positive detection of the wild-type Lep allele is achieved by the use of primer sequence which introduces an A-->C substitution, creating an Msp I site in the normal allele; and an electrophoretic assay which positively identifies the heterozygote.

Source: Obesity Research

DOI: 10.1002/j.1550-8528.1997.tb00291.x

Authors: Wendy K. Chung, Streamson C. Chua, Gwo‐Hwa Lee, Rudolph L. Leibel

Institutions: Rockefeller University, Howard Hughes Medical Institute

Language: English

Topic: Regulation of Appetite and Obesity

Subfield: Endocrine and Autonomic Systems

Field: Neuroscience

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases, Eunice Kennedy Shriver National Institute of Child Health and Human Development