Work

In vitrouptake of amphiphilic, hydrogel nanoparticles by J774A.1 cells

Year: 2009

Type: article

Abstract: We here report improved synthesis and in vitro interactions of amphiphilic hydrogel nanoparticles with the macrophage cell line J774A.1. Nanoparticles comprising dispersed hydrophobic nanodomains of poly(propylene glycol) within a continuous phase of hydrophilic poly (ethylene glycol) (PEG) were prepared via inverse emulsion crosslinking polymerization, using acrylated PEG and Pluronic F127 as macromonomer blocks. Functionality and fluorescent labeling were achieved through incorporation of reactive comonomers and a posteriori reaction with fluorescein, respectively. When introduced to a static cell culture of adhered J774A.1 macrophages, the cells internalized these hydrogel nanoparticles in a dose- and time- dependent manner through clathrin-mediated and other pathways. Amphiphilic nanoparticle uptake was however dramatically lower than that of a model system (Fluospheres) and similar to PEG-coated colloids reported in the literature, which are considered "stealth." Our findings support the potential of the nanoparticles presented here as long-circulating drug carriers.

Source: Journal of Biomedical Materials Research Part A

DOI: 10.1002/jbm.a.32648

Authors: Dimitris Missirlis, Jeffrey A. Hubbell

Institutions: University of California, Santa Barbara, École Polytechnique Fédérale de Lausanne

Language: English

Topic: Nanoparticle-Based Drug Delivery

Subfield: Biomaterials

Field: Materials Science

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W2025328147

FWCI: 0.732

Cites: 39

Cited by: 10

Related to: 10