Regulation of methionine synthesis in Escherichia coli : Effect of metJ gene product and S -adenosylmethionine on the expression of the metF gene
Year: 1985
Type: article
Abstract: The regulation of the expression of the Escherichia coli metF gene, which codes for 5,10-methylenetet-rahydrofolate reductase (EC 1.1.99.15), has been investigated by using a simplified DNA-directed in vitro system that measures the formation of the first dipeptide (fMet-Ser) of the gene product. The synthesis of fMet-Ser directed by a plasmid containing the metF gene is specifically inhibited by metJ protein (repressor protein). S-Adenosylmethionine enhances the inhibition by the metJ protein of metF gene expression. The inhibition by the metJ protein is at the level of transcription and the results suggest that S-adenosylmethionine is functioning as an allosteric effector.
Authors: Robert Shoeman, Betty Redfield, Timothy Coleman, Ronald C. Greene, Albert A. Smith, Nathan Brot, Herbert Weissbach
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2025485768