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Fluorescent Structural DNA Nanoballs Functionalized with Phosphate-Linked Nucleotide Triphosphates

Year: 2010

Type: article

Abstract: Highly labeled DNA nanoballs functionalized with phosphate-linked nucleotide triphosphates (dNTPs) were developed as a source of dNTPs for DNA polymerase. The particles were prepared by strand-displacement polymerization from a self-complementary circular template. Imaged by atomic force microscopy, these functionalized particles appear as condensed fuzzy balls with diameters between 50 and 150 nm. They emit a bright fluorescent signal, detected in 2 ms exposures with a signal-to-noise ratio of 25 when imaged using a TIR fluorescence microscope.

Source: Nano Letters

DOI: 10.1021/nl9039718

Authors: Jon P. Anderson, Bambi L. Reynolds, Kristin Baum, John G. Williams

Institution: LI-COR Biosciences (United States)

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Human Genome Research Institute

Award: Single-Molecule DNA Sequencing Using Charge-Switch dNTPs