Evaluation of ciprofloxacin and metronidazole encapsulated biomimetic nanomatrix gel on Enterococcus faecalis and Treponema denticola
Year: 2015
Type: article
Abstract: Background A triple antibiotic mixture (ciprofloxacin; CF, metronidazole; MN, and minocycline; MC) has been used for dental root canal medicaments in pulp regeneration therapy. However, tooth discolorations, cervical root fractures, and inadequate pulp-dentin formation have been reported due to the triple antibiotic regimen. Therefore, an antibiotic encapsulated biomimetic nanomatrix gel was developed to minimize the clinical limitations and maximize a natural healing process in root canal infections. In this study, minimal bacterial concentrations (MBC) of the selected antibiotics (CF and MN) were tested in 14 representative endodontic bacterial species. Then MBC of each CF and MN were separately encapsulated within the injectable self-assembled biomimetic nanomatrix gel to evaluate antibacterial level on Enterococcus faecalis and Treponema denticola . Results Antibiotic concentrations lower than 0.2 μg/mL of CF and MN demonstrated antibacterial activity on the 14 endodontic species. Furthermore, 6 different concentrations of CF and MN separately encapsulated with the injectable self-assembled biomimetic nanomatrix gel demonstrated antibacterial activity on Enterococcus faecalis and Treponema denticola at the lowest tested concentration of 0.0625 μg/mL. Conclusions These results suggest that each CF and MN encapsulated within the injectable self-assembled biomimetic nanomatrix gel demonstrated antibacterial effects, which could be effective for the root canal disinfection while eliminating MC. In the long term, the antibiotic encapsulated injectable self-assembled biomimetic nanomatrix gel can provide a multifunctional antibiotic delivery method with potential root regeneration. Further studies are currently underway to evaluate the effects of combined CF and MN encapsulated within the injectable self-assembled biomimetic nanomatrix gel on clinical samples.
Source: Biomaterials Research
Language: English
Subfield: Oral Surgery
Field: Dentistry
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,700
Funders: National Science Foundation, National Research Foundation, National Institutes of Health, National Heart, Lung, and Blood Institute, National Institute of Biomedical Imaging and Bioengineering, National Institute of Dental and Craniofacial Research, Division of Chemical, Bioengineering, Environmental, and Transport Systems
Awards: Nitric Oxide Releasing Biomimetic Nanomatrix Gel for Root Revitalization, Prohealing multifunctional endothelium nanomatrix coated stent, G3495671815, G4127891674, A hybrid nanosack for the enhanced islet engraftment in the omentum, CAREER:The Bioactive Hybrid Nanomatrix for Intervertebral Disk Regeneration, Training Program in Cardiovascular Pathophysiology
OpenAlex ID: W2025845360