Work

Replacement of the deoxycytidine residues in Rhizobium bacteriophage RL38JI DNA

Year: 1985

Type: article

Abstract: Rhizobium phage RL38JI DNA is resistant to cleavage by a variety of restriction endonucleases, and is only partially sensitive to digestion by pancreatic DNase I or by micrococcal nuclease. We have found that a mixture of DNase I, P1 nuclease, and bacterial alkaline phosphatase will quantitatively digest RL38JI DNA to deoxyribonucleosides. HPLC analysis revealed that dCyd is nearly totally absent among these digestion products, while dGuo, dAdo, and Thd are readily detected. Three additional peaks are always present; their retention properties correspond to no known modified deoxyribonucleosides. Thus it appears that dCyd is replaced in phage RL38JI DNA by as many as 3 different modified residues.

Source: FEBS Letters

DOI: 10.1016/0014-5793(85)80625-6

Authors: David Swinton, Stanley Hattman, Rolf Benzinger, Vicky Buchanan-Wollaston, John Beringer

Institutions: University of Rochester, University of Virginia, BioTechnica (United States), University of Bristol

Language: English

Topic: DNA and Nucleic Acid Chemistry

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: National Science Foundation, National Institute of General Medical Sciences

Awards: G6942631888, Bacteriophage Infective For Fast-Growing Rhizobia