Work

Facile synthesis and altered ionization efficiency of diverse N ε -alkyllysine-containing peptides

Year: 2011

Type: article

Abstract: Protein lysine methylation is a biologically-important posttranslational modification. Given the increased use of alkyllysine-containing peptides to study protein methylation, we describe an efficient solid-phase synthesis to access various alkyllysine-containing peptides. As an exemplary application, we evaluated how ionization efficiency of the alkyllysine peptides affects mass spectrometric analysis.

Source: Chemical Communications

DOI: 10.1039/c1cc14711k

Authors: Debjani Chakraborty, Kabirul Islam, Minkui Luo

Institution: Memorial Sloan Kettering Cancer Center

Language: English

Topic: Chemical Synthesis and Analysis

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of General Medical Sciences

Awards: Enzyme-engineering Approaches to Dissect Protein Methylation Profiles, Profile substrates and inhibitors of protein lysine methyltransferase