An Invertase Inactivator in Maize Endosperm and Factors Affecting Inactivation
Year: 1971
Type: article
Abstract: A protein present in the developing endosperm of maize (Zea mays L.) causes a loss of invertase activity under certain conditions of incubation. This protein, designated an inactivator, inactivates invertase I of maize even in the presence of other proteins. No inactivation of invertase II of maize or yeast invertase has been observed. The inactivator and invertase I are found only in the endosperm. The quantity of inactivator increases in the normal endosperm during development while invertase I activity decreases. However, the altered levels of invertase I activity in several endosperm mutant lines do not result from different quantities of inactivator. The inactivator can decrease invertase I activity during a preincubation period before addition of sucrose; inactivation is noncompetitive. Invertase I activity decreases curvilinearly with an increase in inactivator concentration. At high buffer concentrations or low inactivator concentrations in the reaction mixture, a latent period is observed when invertase I is not inactivated. Inactivation increases with an increase in temperature and a decrease in pH.
Source: PLANT PHYSIOLOGY
DOI: 10.1104/pp.47.5.629
Authors: T. A. Jaynes, O. E. Nelson
Institution: Purdue University West Lafayette
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: green
OpenAlex ID: W2026333093