Preconditioning with Rapamycin and CTLA4-Ig in Dogs Given Donor Antigen and 1Gy Total Body Irradiation (TBI) Before Dog-Leukocyte-Antigen (DLA)-Identical Marrow Transplantation Failed to Assure Sustained Engraftment
Year: 2012
Type: article
Abstract: Stable engraftment of allogeneic, DLA-identical marrow can be achieved by conditioning dogs with a nonmyeloablative dose of 2 Gy TBI followed by a brief course of postgrafting immunosuppression with cyclosporine (CSP) and mycophenolate mofetil (MMF). However, when TBI dose was reduced to 1 Gy, CSP/MMF failed to control host-versus-graft (HVG) reactions, and grafts were uniformly rejected. CTLA4-Ig blocks T-cell costimulation through the B7:CD28 signaling pathway and induces T cell anergy. We used CTLA4-Ig concurrently with infusion of donor peripheral blood mononuclear cells (PMBC) to blunt activated recipient T cells before transplantation (Storb et al., Blood 94, p2523-2529, 1999).
Institutions: University of Washington, Fred Hutch Cancer Center
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $3,650
OpenAlex ID: W2026526706