Work

Single-Cell Enumeration of an Uncultivated TM7 Subgroup in the Human Subgingival Crevice

Year: 2003

Type: article

Abstract: Specific oligonucleotide hybridization conditions were established for single-cell enumeration of uncultivated TM7 and IO25 bacteria by using clones expressing heterologous 16S rRNA. In situ analysis of human subgingival crevice specimens revealed that a greater proportion of samples from sites of chronic periodontitis than from healthy sites contained TM7 subgroup IO25. In addition, IO25 bacterial cells from periodontitis site samples were more abundant and fourfold longer than IO25 cells from healthy site samples.

Source: Applied and Environmental Microbiology

DOI: 10.1128/aem.69.10.6294-6298.2003

Authors: Cleber C. Ouverney, Gary C. Armitage, David A. Relman

Institutions: University of California, San Francisco, VA Palo Alto Health Care System, Stanford Medicine, Stanford University

Language: English

Topic: Oral microbiology and periodontitis research

Subfield: Periodontics

Field: Dentistry

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funders: University of California, San Francisco, National Institute of Allergy and Infectious Diseases, National Institute of Dental and Craniofacial Research