Year: 2012
Type: article
Abstract: The human nuclear factor related to kappa-B-binding protein (NFRKB) is a 1299-residue protein that is a component of the metazoan INO80 complex involved in chromatin remodeling, transcription regulation, DNA replication and DNA repair. Although full length NFRKB is predicted to be around 65% disordered, comparative sequence analysis identified several potentially structured sections in the N-terminal region of the protein. These regions were targeted for crystallographic studies, and the structure of one of these regions spanning residues 370-495 was determined using the JCSG high-throughput structure determination pipeline. The structure reveals a novel, mostly helical domain reminiscent of the winged-helix fold typically involved in DNA binding. However, further analysis shows that this domain does not bind DNA, suggesting it may belong to a small group of winged-helix domains involved in protein-protein interactions.
Source: PLoS ONE
Authors: Abhinav Kumar, Sabine Möcklinghoff, Fumiaki Yumoto, Lukasz Jaroszewski, Carol L. Farr, Anna Grzechnik, Phuong Nguyen, Christian X. Weichenberger, Hsiu-Ju Chiu, Heath E. Klock, Marc-André Elsliger, Ashley M. Deacon, Adam Godzik, Scott A. Lesley, Bruce R. Conklin, Robert J. Fletterick, Ian A. Wilson
Institutions: SLAC National Accelerator Laboratory, Joint Center for Structural Genomics, Stanford Synchrotron Radiation Lightsource, University of California, San Francisco, Gladstone Institutes, Sanford Burnham Prebys Medical Discovery Institute, Scripps Research Institute, Genomics Institute of the Novartis Research Foundation, University of California San Diego
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,382
Awards: G113944070, G1804828992, G1831385526, G1984473900, G2300450775, G3629260272, G4047395780, G4280418065, G5368254702, Structures of Protein Complexes Regulating Transcription in Enbryonic Stem Cells, G7891900676, A Synchroton Radiation Structural Biology Resource, G9000028044, G9000069621, G9000140088, G9000331358
OpenAlex ID: W2027847853