Work

Novel Design for Centrifugal Countercurrent Chromatography: I. Zigzag Toroidal Column

Year: 2009

Type: article

Abstract: The toroidal coil using an equilateral triangular core has improved both retention of the stationary phase and peak resolution of the conventional toroidal coil in centrifugal countercurrent chromatography. To further improve the retention of stationary phase and peak resolution, a novel zigzag toroidal coil was designed and the performance of the system was evaluated at various flow rates. The results indicated that both retention of stationary phase and peak resolution were improved as the flow rate was decreased. Modification of the tubing by pressing at given intervals with a pair of pliers improved the peak resolution without increasing the column pressure. All these separations were performed under low column pressure indicating the separation can be further improved by increasing the column length and/or revolution speed without damaging the separation column.

Source: Journal of Liquid Chromatography & Related Technologies

DOI: 10.1080/10826070903126856

Authors: Yi Yang, Haji Akber Aisa, Yoichiro Ito

Institutions: National Institutes of Health, Chinese Academy of Sciences, Xinjiang Technical Institute of Physics & Chemistry, National Heart, Lung, and Blood Institute, University of Chinese Academy of Sciences

Language: English

Topic: Chromatography in Natural Products

Subfield: Analytical Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funders: National Institutes of Health, National Heart, Lung, and Blood Institute

Awards: G4705346009, Novel column design for centrifugal countercurrent chromatography