Work

Interaction of α subunit of GTP‐binding protein Go with a 20‐kDa Triton‐insoluble membrane protein in bovine brain

Year: 1992

Type: article

Abstract: Heterotrimeric Go bound to the membranes of bovine brain, but Go alpha remained bound to the membranes even after activation with GTP gamma S. Furthermore, Go alpha bound to a Triton X-100-insoluble fraction of the membranes in a saturable manner. However, the 37-kDa Go alpha eliminated by trypsin at the amino-terminus could not bind to the fraction. Using a blot overlay approach of the Triton-insoluble fraction, only a 20-kDa protein was identified that interacts with Go alpha. These results indicate that Go alpha binds to a 20-kDa Triton-insoluble protein in the bovine brain membranes.

Source: FEBS Letters

DOI: 10.1016/0014-5793(92)81453-s

Authors: Tateki Shiozaki, Manabu Negishi, Satoru Takahashi, Atsushi Ichikawa

Institution: Kyoto University

Language: English

Topic: Receptor Mechanisms and Signaling

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

OpenAlex ID: W2028703402

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Cites: 11

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Related to: 10