Work

Experimental and numerical analysis of DNA nanostrand array formation by molecular combing on microwell-patterned surface

Year: 2008

Type: article

Abstract: DNA molecules can be stretched and immobilized onto a solid surface containing surface microstructures, to form a well-defined array by dewetting the surface with DNA solution. However, the mechanism of DNA stretching, immobilizing and patterning in this molecular combing process is not well understood. In this study, we demonstrated the generation of stretched DNA nanostrand arrays with different orientations and morphologies by controlling the dewetting direction on a microwell-patterned surface. We also simulated the dewetting process based on a deforming body-fitted grid approach. The simulation results provide insights for explaining the stretching, immobilizing and patterning of DNA molecules observed in the experiments.

Source: Journal of Physics D Applied Physics

DOI: 10.1088/0022-3727/42/2/025303

Authors: C H Lin, J Guan, S W Chau, L J Lee

Institutions: Florida State University, Florida A&M University - Florida State University College of Engineering, National Taiwan University of Science and Technology, The Ohio State University

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funder: National Science Council

OpenAlex ID: W2029360676

FWCI: 0.124

Cites: 14

Cited by: 9

Related to: 10