Fixation of Neural Tissues for Electron Microscopy by Perfusion With Solutions of Osmium Tetroxide
Year: 1962
Type: article
Abstract: This paper describes in detail a method for obtaining nearly uniform fixation of the nervous system by vascular perfusion with solutions of osmium tetroxide. Criteria are given for evaluating the degree of success achieved in the preservation of all the cellular components of the nervous system. The method permits analysis of the structural relations between cells at the electron microscopic level to an extent that has not been possible heretofore.
Source: The Journal of Cell Biology
DOI: 10.1083/jcb.12.2.385
Authors: Sanford L. Palay, S. M. McGee-Russell, Spencer Gordon, Mary A. Grillo
Institution: National Institutes of Health
Language: English
Subfield: Anatomy
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2029375622