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Inactivation of D-Alanine Carboxypeptidase by Penicillins and Cephalosporins Is Not Lethal in Bacillus subtilis

Year: 1971

Type: article

Abstract: The D-alanine carboxypeptidase of Bacillus subtilis is a particulate enzyme that is irreversibly inactivated by penicillins and cephalosporins. However, the lethal concentrations of these antibiotics are not the same as those that inhibit enzymatic activity in vitro. 6-Aminopenicillanic acid inactivates at least 95% of the enzyme at nonlethal concentrations. Conversely, cephalothin is lethal at concentrations that do not inactivate the enzyme. Experiments with intact, growing cells confirm the results obtained in vitro. Therefore, a killing site distinct from the carboxypeptidase must be postulated.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.68.11.2814

Authors: Peter M. Blumberg, Jack L. Strominger

Institution: Harvard University

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Science Foundation, U.S. Public Health Service

OpenAlex ID: W2030330409

FWCI: 7.923

Cites: 6

Cited by: 73

Related to: 10