Evaluation of Trichoderma harzianum and Gliocladium roseum in controlling leaf blotch of wheat (Septoria tritici) under in vitro and greenhouse conditions
Year: 1997
Type: article
Abstract: The suitability of Trichoderma harzianum and Gliocladium roseum as biocontrol agents on the in vitro growing of Septoria tritici and their efficiency in reducing disease severity on wheat plants was evaluated under greenhouse conditions. For the in vitro tests, the micro-organisms were confronted through dual cultures on PDA. A histopathological clarification and staining technique was used in order to observe, if the penetration and development of the pathogen on wheat leaves was modified in the presence of the antagonistic agents. A highly significant effect of T. harzianum and G. roseum on the development of S'. tritici colonies was observed. Both antagonistic agents grew over the S. tritici colony and covered it completely. T. harzianum was more efficient than G. roseum in inhibiting the pathogen. The coiling of the hypha of T. harzianum on short sections of S. tritici colony was observed with the bioassay on wheat leaves. The spore germination and the superficial mycelial development of S. tritici was affected by T. harzianum and G. roseum. Although, the further stomatic penetration of S. tritici was not restrained. The in vivo inhibitory capacity of the two antagonists assayed were not expressed on the wheat plants tested. However, there has been a tendency to reduce the percentage of necrotic area with pycnidial coverage when compared with the control. Further studies are needed before unequivocal conclusions can be drawn. Greenhouse experiments are in progress.
Language: German
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: green
OpenAlex ID: W203035271