Work

Thioamide quenching of intrinsic protein fluorescence

Year: 2011

Type: article

Abstract: Thioamides quench tryptophan and tyrosine fluorescence in a distance-dependent manner and thus can be used to monitor the binding of thioamide-containing peptides to proteins. Since thioamide analogs of the natural amino acids can be synthetically incorporated into peptides, they can function as minimally-perturbing probes of protein/peptide interactions.

Source: Chemical Communications

DOI: 10.1039/c1cc14708k

Authors: Jacob M. Goldberg, Rebecca F. Wissner, Alyssa M. Klein, E. James Petersson

Institution: University of Pennsylvania

Language: English

Topic: Protein Interaction Studies and Fluorescence Analysis

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institute of General Medical Sciences

Awards: Predoctoral Training Program in Genetics, Predoctoral Training at the Chemistry-Biology Interface