Work

One-dimensional topography underlies three-dimensional fibrillar cell migration

Year: 2009

Type: article

Abstract: Current concepts of cell migration were established in regular two-dimensional (2D) cell culture, but the roles of topography are poorly understood for cells migrating in an oriented 3D fibrillar extracellular matrix (ECM). We use a novel micropatterning technique termed microphotopatterning (microPP) to identify functions for 1D fibrillar patterns in 3D cell migration. In striking contrast to 2D, cell migration in both 1D and 3D is rapid, uniaxial, independent of ECM ligand density, and dependent on myosin II contractility and microtubules (MTs). 1D and 3D migration are also characterized by an anterior MT bundle with a posterior centrosome. We propose that cells migrate rapidly through 3D fibrillar matrices by a 1D migratory mechanism not mimicked by 2D matrices.

Source: The Journal of Cell Biology

DOI: 10.1083/jcb.200810041

Authors: Andrew D. Doyle, Francis W. Wang, Kazue Matsumoto, Kenneth M. Yamada

Institutions: National Institutes of Health, National Institute of Dental and Craniofacial Research, National Institute of Standards and Technology

Language: English

Topic: Cellular Mechanics and Interactions

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Funder: National Institutes of Health

OpenAlex ID: W2030582971

FWCI: 16.62

Cites: 26

Cited by: 757

Related to: 10