Work

A Primer for Morpholino Use in Zebrafish

Year: 2009

Type: article

Abstract: Morpholino oligonucleotides are the most common anti-sense "knockdown" technique used in zebrafish (Danio rerio). This review discusses common practices for the design, preparation, and deployment of morpholinos in this vertebrate model system. Off-targeting effects of morpholinos are discussed as well as method to minimize this potentially confounding variable via co-injection of a tP53-targeting morpholino. Finally, new uses of morpholinos are summarized and contextualized with respect to the complementary, DNA-based knockout technologies recently developed for zebrafish.

Source: Zebrafish

DOI: 10.1089/zeb.2008.0555

Authors: Brent R. Bill, Andrew M. Petzold, Karl J. Clark, Lisa A. Schimmenti, Stephen C. Ekker

Institutions: University of Minnesota, Mayo Clinic

Language: Indonesian

Topic: Zebrafish Biomedical Research Applications

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Funders: National Eye Institute, National Institute of General Medical Sciences

Awards: Visual Neuroscience Training Program, Systematic Vertebrate Functional Genomics, Systematic Vertebrate Functional Genomics