Work

Parallel human genome analysis: microarray-based expression monitoring of 1000 genes.

Year: 1996

Type: article

Abstract: Microarrays containing 1046 human cDNAs of unknown sequence were printed on glass with high-speed robotics. These 1.0-cm2 DNA "chips" were used to quantitatively monitor differential expression of the cognate human genes using a highly sensitive two-color hybridization assay. Array elements that displayed differential expression patterns under given experimental conditions were characterized by sequencing. The identification of known and novel heat shock and phorbol ester-regulated genes in human T cells demonstrates the sensitivity of the assay. Parallel gene analysis with microarrays provides a rapid and efficient method for large-scale human gene discovery.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.93.20.10614

Authors: M Schena, D Shalon, R Heller, A Chai, P O Brown, R W Davis

Institution: Stanford Medicine

Language: English

Topic: Gene expression and cancer classification

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Science Foundation, Howard Hughes Medical Institute, National Institutes of Health, National Human Genome Research Institute

Awards: EFFICIENT GENE MAPPING BY WHOLE GENOME MISMATCH SCANNING, CELL REGULATION: BIOCHEMICALLY ISOLATED DNA SEGMENTS, Development of Molecular Biology Approaches to Arabidopsis thaliana