Work

Mammalian cell fusion in an electroporation device

Year: 1995

Type: article

Abstract: We and others have been interested in the phenomenon of gene 'extinction' in somatic cell hybrids, reasoning that the study of this process is likely to reveal underlying mechanisms responsible for limiting the expression of specialized genes only to appropriate cell types. In the course of our studies in this area, we have developed a simple and economical method of fusing mammalian cells, using an electroporation device. In fusions between murine myeloma and T lymphoma lines, hybrid cell recoveries were typically one per 10(5) [corrected] input myeloma cells. Because of our interest in the regulation of immunoglobulin heavy chain (IgH) gene expression, we analyzed the hybrids for both IgH gene composition and expression. The hybrid lines were phenotypically indistinguishable from those generated by the more conventional, polyethylene glycol (PEG)-induced fusion protocol. There was a notable increase, however, in the number of hybrids that retained IgH-encoding chromosomes from both parental lines.

Source: Journal of Immunological Methods

DOI: 10.1016/0022-1759(95)00205-7

Authors: Hanna S. Radomska, Laurel A. Eckhardt

Institutions: City University of New York, Hunter College, Columbia University

Language: English

Topic: Microbial Inactivation Methods

Subfield: Biotechnology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $2,970

Funder: National Cancer Institute