Work

Direct isolation of human BRCA2 gene by transformation-associated recombination in yeast

Year: 1997

Type: article

Abstract: Mutant forms of the BRCA2 gene contribute significantly to hereditary breast cancer. Isolation of the normal and mutant forms of the BRCA2 gene with its natural promoter would greatly facilitate analysis of the gene and its contribution to breast cancer. We have accomplished the direct isolation of the 90-kb gene from total human DNA by transformation-associated recombination in yeast using a small amount of 5' and 3' BRCA2 sequence information. Because the entire isolation procedure of a single chromosomal gene could be accomplished in approximately 2 weeks, the transformation-associated recombination cloning approach is readily applicable to studies of chromosome alterations and human genetic diseases.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.94.14.7384

Authors: Vladimir Larionov, Natalya Kouprina, Gregory Solomon, J. Carl Barrett, Michael A. Resnick

Institutions: National Institutes of Health, National Institute of Environmental Health Sciences

Language: English

Topic: Fungal and yeast genetics research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W2031052993

FWCI: 1.662

Cites: 19

Cited by: 95

Related to: 10