Details of ssDNA annealing revealed by an HSV-1 ICP8–ssDNA binary complex
Year: 2013
Type: article
Abstract: Infected cell protein 8 (ICP8) from herpes simplex virus 1 was first identified as a single-strand (ss) DNA-binding protein. It is essential for, and abundant during, viral replication. Studies in vitro have shown that ICP8 stimulates model replication reactions, catalyzes annealing of complementary ssDNAs and, in combination with UL12 exonuclease, will catalyze ssDNA annealing homologous recombination. DNA annealing and strand transfer occurs within large oligomeric filaments of ssDNA-bound ICP8. We present the first 3D reconstruction of a novel ICP8-ssDNA complex, which seems to be the basic unit of the DNA annealing machine. The reconstructed volume consists of two nonameric rings containing ssDNA stacked on top of each other, corresponding to a molecular weight of 2.3 MDa. Fitting of the ICP8 crystal structure suggests a mechanism for the annealing reaction catalyzed by ICP8, which is most likely a general mechanism for protein-driven DNA annealing.
Source: Nucleic Acids Research
DOI: 10.1093/nar/gkt266
Language: English
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,992
Awards: G2729027624, G2774293502, DNA-Protein Interactions at the Replication Fork, G5668739698, Resource for Biocomputing, Visualization, and Informatics, SUPPORT AND DEVELOPMENT OF EMAN FOR ELECTRON MICROSCOPY IMAGE PROCESSING, Nucleoprotein Structures at Telomeres and Sites of DNA Damage, G8249830121, G9000015997, G9000271690, Viral Oncogenesis, Latency, and Replication, Cancer Center Support Grant
OpenAlex ID: W2031135174