Work

Prostaglandin E receptor EP3γ isoform, with mostly full constitutive Gi activity and agonist‐dependent Gs activity

Year: 1996

Type: article

Abstract: We recently demonstrated that two exclusively Gi-coupled isoforms of the mouse EP3 receptor, EP3alpha and beta, with different carboxyl-terminal tails, differed in agonist-independent constitutive Gi activity, and the carboxyl-terminal tail-truncated receptor showed full constitutive activity (Hasegawa, H., Negishi, M., and Ichikawa, A. (1996) J. Biol. Chem. 271, 1857-1860). Here we further examined Gi and Gs activities of the third isoform, EP3gamma, coupled to both Gi and Gs. The My receptor showed mostly full constitutive Gi activity and agonist-dependent Gs activity. The truncated receptor also showed agonist-dependent Gs activity, but the level was lower than that of the EP3gamma receptor. Thus, the carboxyl-terminal tail would differentially regulate Gi and Gs activities of the EP3 receptor.

Source: FEBS Letters

DOI: 10.1016/0014-5793(96)00354-7

Authors: Manabu Negishi, Hiroshi Hasegawa, Atsushi Ichikawa

Institution: Kyoto University

Language: English

Topic: Receptor Mechanisms and Signaling

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: Life Sciences Research Foundation, Japan Society for the Promotion of Science

Awards: Pharmaceutical approaches using prostanoid receptor knockout mice, MOLECULAR APPROACHES FOR FUNCTIONS AND PHYSIOLOGICAL SIGNIFICANCE OF PROSTAGLANDIN E RECEPTORS