Work

Increased oxidative stress in the RAW 264.7 macrophage cell line is partially mediated via the S‐nitrosothiol‐induced inhibition of glutathione reductase

Year: 1999

Type: article

Abstract: We investigated whether endogenously or exogenously produced nitric oxide (NO) can inhibit cellular glutathione reductase (GR) via the formation of S-nitrosothiols to decrease cellular glutathione (GSH) and increase oxidative stress in RAW 264.7 cells. The specificity of this inhibition was demonstrated by addition of a NO-synthase inhibitor, and met- or oxyhemoglobin. Using isolated GR we found that only certain NO donors inhibit this enzyme via S-nitrosothiol. Furthermore, we found that cellular GSH decrease is paralleled by an increase of superoxide anion production. Our results show that the GR enzyme is a potential target of S-nitrosothiols to decrease cellular GSH levels and to induce oxidative stress in macrophages.

Source: FEBS Letters

DOI: 10.1016/s0014-5793(99)00139-8

Authors: Urwe Butzer, Hans Weidenbach, Susanne Gansauge, Frank Gansauge, Hans G Beger, Andreas K Nussler

Institution: Universität Ulm

Language: English

Topic: Redox biology and oxidative stress

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: Bundesministerium für Bildung und Forschung, Universität Ulm, National Cancer Institute