Work

Kinetics of Wnt-Driven β-Catenin Stabilization Revealed by Quantitative and Temporal Imaging

Year: 2008

Type: article

Abstract: The Wnt/beta-catenin signal transduction pathway regulates a broad range of developmental processes. Aberrant activation of the Wnt pathway leads to cancer and degenerative diseases. beta-catenin is a key signaling molecule that is frequently used as a direct monitor of Wnt pathway activation. This paper describes a multi-parametric method for quantitative analysis of cellular beta-catenin protein levels in a rapid and high-throughput manner. The assay offers temporally resolved detection of Wnt-stimulated accumulation of beta-catenin, simultaneously detecting cell number, and it sheds light onto the kinetics of posttranslational stabilization of beta-catenin.

Source: PLoS ONE

DOI: 10.1371/journal.pone.0003498

Author: Rami N. Hannoush

Institution: Genentech

Language: English

Topic: Wnt/β-catenin signaling in development and cancer

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,382

Funder: Genentech