Crystallization and preliminary X-ray diffraction analysis of a soluble domain of the putative zinc transporter CzrB fromThermus thermophilus
Year: 2007
Type: article
Abstract: CzrB is a putative zinc transporter from Thermus thermophilus. The protein is proposed to consist of a hexahelical transmembrane domain with a cytosolic extramembranal C-terminus. The latter 92-residue fragment may be expressed free and may function independently of the full-length integral membrane protein. A 6xHis-tagged form of the water-soluble fragment has been overexpressed in Escherichia coli and diffraction-quality crystals of the tagged and tag-free variants have been grown. Preliminary X-ray analyses of tag-free fragment crystals with (2.2 A resolution) and without zinc ions (1.7 A resolution) reveal that the former has at least two zinc ions bound per monomer.
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Open Access status: bronze
Awards: Robotic Dispensing of Cubic Phase for Crystallizing Membrane Proteins, MEMBRANE PROTEIN STRUCTURE : USE OF LIPIDIC MESOPHASES, G1641640346, Operation of the Cornell High Energy Synchrotron Radiation Laboratory (CHESS), Membrane Proteins, Crystallogenesis and X-ray Structure, G2728615258, Macromolecular Diffraction Resource: MacCHESS, The Structural Biology of Membrane Proteins: Proteomics via Crystal Structure, G5781590026, G705610926, G7516500822, G9000231267
OpenAlex ID: W2032174270