Work

Extension of the lifespan of cultured normal human diploid cells by vitamin E: a reevaluation.

Year: 1977

Type: article

Abstract: Previously we reported [Packer, L. & Smith, J.R. (1974) Proc. Natl. Acad. Sci. USA 71, 4763-4767] that the lifespan of WI-38 human diploid fibroblasts in vitro was significantly increased by continuously growing the cell cultures in the presence of vitamin E(dl-alpha-tocopherol), but in 19 subsequent subcultivation series we were unable to reproduce these findings. While vitamin E is incorporated into the cells and is able to act effectively as an antioxidant, apparantly is intracellular antioxidant properties alone do not routinely result in an increase of cell lifespan. A synergism between vitamin E and some component(s) in the first of two lots of serum used in the original experiments seems the most likely explanation for our earlier findings.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.74.4.1640

Authors: L Packer, J R Smith

Institutions: Lawrence Berkeley National Laboratory, University of California, Berkeley, W. Alton Jones Cell Science Center

Language: English

Topic: Antioxidant Activity and Oxidative Stress

Subfield: Biochemistry

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funder: U.S. Public Health Service