Work

Antisera of designed specificity for subunits of guanine nucleotide-binding regulatory proteins.

Year: 1986

Type: article

Abstract: Antisera were raised against purified subunits of regulatory GTP-binding proteins (G proteins) and against synthetic peptides that correspond to defined regions of G proteins. Peptide antisera were generated that recognized all alpha or all beta subunits from Gs, Gi, Go, and transducin; others recognized only Gs alpha or Go alpha. Such cross-reaction or complete specificity for a given alpha subunit was not obtained when purified subunits were injected. Peptide antisera were used to identify G protein subunits in selected tissue membrane preparations by immunoblots.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.83.2.265

Authors: S M Mumby, R A Kahn, D R Manning, A G Gilman

Institutions: University of Texas Health Science Center at Dallas, The University of Texas Southwestern Medical Center

Language: English

Topic: Receptor Mechanisms and Signaling

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: U.S. Public Health Service, National Institute of General Medical Sciences

Award: G976133492