Work

Modified RNA sequence pools forin vitroselection

Year: 1994

Type: article

Abstract: We report the use of modified RNA, in which the 2'-OH group of pyrimidines is replaced by a 2'-amino (2'-NH2) group to identify high affinity ligands specific for human neutrophil elastase (HNE) by in vitro selection. Compared to unmodified RNA the 2'-NH2-modified RNA ligands show enhanced stability in human serum and urine. Use of RNase T1 cleavage data in the presence of K+ and Li+ ions suggests that the modified RNA ligands selected for HNE form an intermolecular G-quartet structure.

Source: Nucleic Acids Research

DOI: 10.1093/nar/22.24.5229

Authors: Yun Lin, Qiu Qiu, Stanley C. Gill, Sumedha D. Jayasena

Language: English

Topic: DNA and Nucleic Acid Chemistry

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2033231386

FWCI: 3.61

Cites: 39

Cited by: 188

Related to: 10