Year: 2011
Type: article
Abstract: Fucan is a term used to denominate a family of sulfated polysaccharides rich in sulfated l-fucose. Heterofucan SF-1.5v was extracted from the brown seaweed Sargassum filipendula by proteolytic digestion followed by sequential acetone precipitation. This fucan showed antiproliferative activity on Hela cells and induced apoptosis. However, SF-1.5v was not able to activate caspases. Moreover, SF-1.5v induced glycogen synthase kinase (GSK) activation, but this protein is not involved in the heterofucan SF-1.5v induced apoptosis mechanism. In addition, ERK, p38, p53, pAKT and NFκB were not affected by the presence of SF-1.5v. We determined that SF-1.5v induces apoptosis in HeLa mainly by mitochondrial release of apoptosis-inducing factor (AIF) into cytosol. In addition, SF-1.5v decreases the expression of anti-apoptotic protein Bcl-2 and increased expression of apoptogenic protein Bax. These results are significant in that they provide a mechanistic framework for further exploring the use of SF-1.5v as a novel chemotherapeutics against human cervical cancer.
Source: Marine Drugs
DOI: 10.3390/md9040603
Authors: Leandro Silva Costa, Cinthia Beatrice Silva Telles, Ruth Medeiros Oliveira, Leonardo Thiago Duarte Barreto Nobre, Nednaldo Dantas-Santos, Rafael Barros Gomes Camara, Mariana Santana Santos Pereira Costa, Jailma Almeida-Lima, Raniere Fagundes Melo-Silveira, Ivan Rui Lopes Albuquerque, Edda Lisboa Leite, Hugo Alexandre Oliveira Rocha
Language: English
Subfield: Aquatic Science
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,499
OpenAlex ID: W2033365400